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. 2021 Sep 24;10(10):2005. doi: 10.3390/plants10102005

Figure 1.

Figure 1

Variable nucleotides within the psbA-trnH mini-barcode (voucher information is in Table 1; herbal dietary supplement information is in Table 2; the full alignment is in Figure A1). Alignment positions are numbered vertically. Bases identical to the first sequence are indicated with “.”. Variable bases are indicated with standard International Union of Pure and Applied Chemistry (IUPAC) codes: D = {AGT}, K = {GT}, M = {AC}, N = {ACGT}, and W = {AT}. The number of sequences summarized (n) for each species/supplement type is indicated. Alignment positions that unambiguously distinguish Harpagophytum from all other Pedaliaceae (16, 64, and 116) are highlighted in blue. The alignment positions 76 and 107—which distinguish between the two Harpagophytum species—are highlighted in orange.