Table 4.

PCR cycling conditions used. Amplification reactions used an initial denaturation of 150 s at 95 °C and a final extension of 600 s at 72 °C (psbA-trnH used 64 °C). Primer names correspond to those in Table 3.

Marker	Primers	Cycling
matK	1R & 3F	10 × {30 s, 95 °C; 30 s, 56 °C; 30 s, 72 °C}; 25 × {30 s, 88 °C; 30 s, 56 °C; 30 s, 72 °C}
nrITS2	S2F & S3R	35 × {30 s, 95 °C; 30 s, 56 °C; 30 s, 72 °C}
psbA-trnH	psbAF & trnHR	10 × {30 s, 95 °C; 120 s, 55 °C}; 23 × {45 s, 90 °C; 120 s, 55 °C}
psbA-trnH mini-barcode	F & R	35 × {30 s, 95 °C; 120 s, 58 °C}
rbcL	32F & ajf634R	35 × {30 s, 95 °C; 30 s, 58 °C; 30 s, 72 °C}
rbcL	a_F & ajf634R	35 × {30 s, 95 °C; 30 s, 58 °C; 30 s, 72 °C}