FIG. 9.

Smt3 processing and Smt3-protein conjugates in ulp mutants. (A) Suppression of ulp2Δ temperature sensitivity by catalytically defective Ulp1 mutants. (B) Smt3-protein conjugates in ulp mutants at 30°C. The strains used were MHY1614 to MHY1617. (C) Reciprocal suppression of ulp2Δ and ubc9-1 growth defects. (D) Provision of additional mature Smt3 does not impair the suppression of benomyl sensitivity in the ulp2Δ ulp1^ts mutant. The YPD plates included 100 μM CuSO₄ to induce His₆-Smt3 expression from the YRTAG310-His6-SMT3-gg plasmid.