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. 2021 Sep 25;10(10):2014. doi: 10.3390/plants10102014

Figure 1.

Figure 1

Sequence alignment and phylogenetic analysis of HcMYB132. (a) Multiple sequence alignment of HcMYB132 with R2R3-MYB proteins. Sequence alignment was performed by ClustalX 2.1 and shaded in GeneDoc. Amino acid residues are shaded in light gray, gray, and black showing 50, 70 and 100% identity, respectively, while dashes indicate gaps used for optimal alignment. R2R3 motifs are indicated by orange lines. (b) Phylogenetic analysis of HcMYB132 together with previously characterized R2R3-MYB proteins. The protein sequences were aligned by Clustal X 2.1 and the phylogenetic tree was built in MEGA X using the Nj method. All R2R3-MYBs are grouped into 4 subclades named G I–G IV. Genes used in phylogenetic tree and their accession numbers are listed in Table S2.