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. 2021 Oct 13;22(20):11026. doi: 10.3390/ijms222011026

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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IHC staining of osteogenic factors BMP-2 of the cross section of the calvarial defects at Week 12 post-operation. Representative BMP-2-stained images of calvarial bone sections from each group: Non-antibody staining control (a), Control (b), DM (c), and DM + PBMT treatment (d). Calvarial bone defects (3 mm) were generated in adult Wistar rats. PBMT treatment (4 J/cm²) was applied daily. The calvarial bone sections were collected at Week 12 postoperatively and stained with BMP-2 antibodies. Scale bar, 1000 μm for 40× and 100 μm for 400×.