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. 2021 Oct 13;22(20):11042. doi: 10.3390/ijms222011042

Figure 4.

Figure 4

Knockdown of SH3BGR affects cell viability and augments apoptosis via hippo signaling- (A) SH3BGR knockdown dramatically reduced the phosphorylation of LATS1 and moderately increased total YAP without altering phosphorylated YAP (pYAP) levels as observed in immunoblots; its densitometric analysis is shown in (B). SH3BGR knockdown elevated active, i.e., cleaved, forms of Caspase 3 and 7 (C,D), indicating increased apoptosis in NRVCMs, which was also reflected in the reduced cell survival determined by cell viability assay (E). Statistical calculations were carried out using a two-tailed Student’s t-test. *, p < 0.05; **, p < 0.01; LATS1, Large tumor suppressor kinase 1; pLATS1, phosphorylated LATS1; YAP, Yes1 Associated Transcriptional Regulator; pYAP, phosphorylated YAP.