Table 4.
Microbiological spermogram before and after the use of hygiene protocol and the biosecurity of sperm collection (HPBC).
| Bacterial Burden (103 CFU/mL) |
Fungal Burden (103 CFU/mL) |
|||
|---|---|---|---|---|
| Raw Semen | Diluted Semen | Raw Semen | Diluted Semen | |
| Before (HPBC) | 62.39 | 0.125 | 0.155 | 0.153 |
| After (HPBC) | 31.29 | 0.016 | 0.140 | 0.139 |
| CFU Evolution (%) | −49.85% | −87.2% | −9.67% | −915% |
| Statistical Significance (p) | <0.00001 (yes) | 0.000726 (yes) | 0.111491 (no) | 0.08078 (no) |
HPBC = Hygiene Protocol and the Biosecurity of sperm Collection. HPBC is highly effective against bacterial contamination of semen—both raw (p < 0.00001) and diluted (p = 0.000726)—but seems to have no effect against fungal burden (p > 0.05).