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. 2021 Sep 26;13(10):1936. doi: 10.3390/v13101936

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Timeline of the immunization and blood collection. BALB/c mice were immunized with high and low doses of RBD-hFc, RBD-mFc and S1-hFc proteins mixed with 0.5 mg/mL aluminum hydroxide adjuvant each and boosted 1 week later. Blood were taken at 1, 2, 3, 4, 5, 6, 8 and 12 weeks after the second vaccination. Spleens were collected after euthanizing.