FIG. 7.
Effect of telomere length on TPE. As for Fig. 1, TPE was monitored in a series of isogenic strains with TELXVL::URA3. (a) TPE in wild-type, mec1-21, rif1, and mec1-21 rif1 strains. Top rows, RCY106-4d; second rows, RCY106-3d; third rows, RCY106-1d; fourth rows, RCY106-14a. (b) Telomere lengths in wild-type, mec1-21, rif1, and mec1-21 rif1 strains. Purified genomic DNA was digested with PstI and examined by Southern analysis using a hybridization probe derived from the Y′ subtelomeric repeats (30). The positions of size standards are shown on the right. Lane 1, RCY106-4d (wild type); lane 2, RCY106-3d (mec1-21); lane 3, RCY106-1d (rif1); lane 4, RCY106-11b (mec1-21 rif1).