TABLE 1.
Haploid strains used in this studya
| Strain | Relevant genotype | Progenitor strain or reference |
|---|---|---|
| W303a | Wild type | 36 |
| RCY50 | TELXVL::URA3 | W303a |
| JMY303-2d | α mec1-21 sml1::HIS3 | 30 |
| JMY303-3b | α sml1::HIS3 | 30 |
| RCY61-1a | rif1 rif2 | RCY61 |
| RCY78-3b | α rif1 mec1-21 | RCY78 |
| RCY106-1d | rif1 TELXVL::URA3 | RCY106 |
| RCY106-3d | mec1-21 TELXVL::URA3 | RCY106 |
| RCY106-4d | TELXVL::URA3 | RCY106 |
| RCY106-14a | rif1 mec1-21 TELXVL::URA3 | RCY106 |
| RCY109-1b | sml1::HIS3 TELXVL::URA3 | RCY109 |
| RCY109-1c | mec1-21 TELXVL::URA3 | RCY109 |
| RCY109-2b | TELXVL::URA3 | RCY109 |
| RCY109-3a | mec1-21 sml1::HIS3 TELXVL::URA3 | RCY109 |
| RCY109-4d | α TELXVL::URA3 | RCY109 |
| RCY109-9b | α TELXVL::URA3 | RCY109 |
| RCY109-25c | α mec1-21 TELXVL::URA3 | RCY109 |
| RCY77 | TELVIILΔ::URA3 | W303a |
| RCY110-1b | α mec1-21 TELVIILΔ::URA3 | RCY110 |
| RCY110-5d | α TELVIILΔ::URA3 | RCY110 |
| LPY253 | hml::TRP1 | 29 |
| JMY303-15c | α mec1-21 | 30 |
| RCY112-5c | hml::TRP1 mec1-21 | RCY112 |
| RCY112-13b | hml::TRP1 mec1-21 | RCY112 |
| RCY84-2c | α rap1-17 | RCY84 |
| RCY124-2a | hml::TRP1 rap1-17 | RCY124 |
| YS148 | chk1Δ::HIS3 rad53-21 | Y. Sanchez and S. Elledge |
| W1973-6b | rad53-1 | X. Zhao and R. Rothstein |
| W1974-6d | rad53-17 | X. Zhao and R. Rothstein |
| RCY126-3c | rad53-21 TELXVL::URA3 | RCY126 |
| RCY126-1d | chk1Δ::HIS3 TELXVL::URA3 | RCY126 |
| DLY298 | rad9Δ::HIS3 | T. Weinert |
| RCY143-2c | rad9Δ::HIS3 TELXVL::URA3 | RCY143 |
| Y286 | α dun1-Δ100::HIS3 | 43 |
| RCY144-4b | dun1-Δ100::HIS3 TELXVL::URA3 | RCY144 |
| DLY339 | mec3::HIS3 | T. Weinert |
| RCY160-1b | α rad9Δ::HIS3 TELXVL::URA3 | RCY160 |
| RCY160-3a | α mec1-21 rad9Δ::HIS3 TELXVL::URA3 | RCY160 |
| RCY160-4b | α mec1-21 TELXVL::URA3 | RCY160 |
| RCY160-4d | α TELXVL::URA3 | RCY160 |
| RCY175-4c | dun1-Δ100::HIS3 sml1::HIS3 TELXVL::URA3 | RCY175 |
a
All strains are isogenic with W303a (a leu2-3,112 his3-11,15 ura3-1 ade2-1 trp1-1 can1-100), except for the markers indicated. The construction of the diploids is described in Materials and Methods.