Figure 4.

Maximum likelihood phylogeny (RAxML v.8, bootstrap values above 75 (1000 replicates) are noted at the nodes) of black fly pools collected in NM between March and November of 2020, 11 voucher specimen sequences, and one outgroup, Liohippelates pusio, inferred from mtDNA CoxI. NM black fly sequences are colored by the month in which they were collected and labeled with the state, the site, and the pool number. Nodes without bootstrap support indicate <70% bootstrap support and the scale bar represents 10% difference between two sequences. The location of collection for voucher specimens is provided in Table 1.