Table 3.
VSIV detections in black fly pools screened using rRT-PCR and results of Sanger sequencing.
| March 2020 | April 2020 | May 2020 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Black Fly Species | Pools Screened 1 |
rRT-PCR Pos. | Ct Range | Sequences Amplified | Pools Screened |
rRT-PCR Pos. | Ct Range | Sequences Amplified | Pools Screened |
rRT-PCR Pos. | Ct Range | Sequences Amplified |
| Simulium sp. | − | − | − | − | 8 | 0 | − | 0 | 13 | 2 (15.4%) | 30.1–32.5 | 0 |
| S. argus | − | − | − | − | 3 | 0 | − | 0 | − | − | − | − |
| S. mediovittatum | − | − | − | − | 5 | 3 (60%) | 30.1–30.4 | 2 (66.7%) | 8 | 3 (37.5%) | 30.1–31.6 | 2 (66.7%) |
| S. meridionale | − | − | − | − | 14 | 0 | − | 0 | 12 | 1 (8.3%) | 31.5 | 0 |
|
S. notatum/ griseum |
− | − | − | − | 1 | 1 (100%) | 29.82 | 1 (100%) | − | − | − | − |
| S. robynae | 1 | 0 | − | 0 | 3 | 0 | − | 0 | 9 | 1 (11.1%) | 27.0 | 0 |
1 Pool size ranged from 1 to 20 black flies. Positive pools were collected from six transects: RG_027, RG_030, RG_031, RG_032, RG_038, RG_040.