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Primers used in PCR amplifications
| Primer | Sequence (5′ to 3′) | Annealing temp (°C) |
|---|---|---|
| MFαU | TTCACTGCCATCTTCACCACC | 55 |
| MFαL | TCTAGGCGATGACACAAAGGG | 55 |
| 660U | TATTGGACTAAAACGGTATGCGG | 55 |
| 660L | CGACGACGAGGTATTCTTTTTCC | 55 |
| STE12αU | CAATCTCAAAGCGGGGACAG | 50 |
| STE12αL | CTTTGTTTCGGTCCTAATACAGCC | 50 |
