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Primers used for quantitative RT-PCR.
| Gene | Forward (5′-3′) | Reverse (5′-3′) |
|---|---|---|
| ACTB | ACACGGTGCCCATCTACGAG | GCTTCTCCTTGATGTCCCGC |
| ZO1 | AGCCATCCACTCCTGCCTAT | GACGGGACCTGCTCATAACT |
| OCLN | CTTTCTCAGCCAGCGTATTC | AGGCAAGCGTGGAGGCAACA |
| CLDN1 | CATTGCTATCTTTGCCTGTG | GCCATAACCGTAGCCATAAC |
| MLCK | CCTGTCCTGGTATGGCTCCT | CTGCGGCATGTGGCTAGTTC |
