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. 2021 Oct 4;13(10):1991. doi: 10.3390/v13101991

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Identification of the rescued PDCoV, rCHN-HN-2014. (A) Indirect immunofluorescence analysis of LLC-PK1 cells infected with rCHN-HN-2014 and CHN-HN-2014 at 12 hpi using anti-PDCoV N monoclonal antibody. Bar, 100 μm. (B) Differentiation between rCHN-HN-2014 and CHN-HN-2014. The presence of a ClaI restriction site resulted in fragments of 364 bp and 716 bp after digestion with this enzyme. As expected, the restriction site was found in CHN-HN-2014 but not in the rCHN-HN-2014. (C) Identification of the introduced molecular marker (15121C→A) in the rCHN-HN-2014 via DNA sequencing. The dotted and red frame represent the molecular marker site and the recognition sequence by ClaI restriction enzymes, respectively.