Table 1.
Primers used for the RT-PCR amplification of PDCoV full-length genome, the construction of plasmids and real-time RT-PCR assay.
| Primer | Nucleotide Sequence (5′-3′) |
|---|---|
| CMV-F: | GGCGTGCACTTGACATTGATTATTGACTAGTTAT |
| CMV-R | TAATTTTTATCTTTAGTCCCCATGTACGGTTCACTAAACGAGCTCTGCTTATATAGACC |
| PDCoV-5′-F | ACATGGGGACTAAAGATAAAAATTATAGCATTAGTCT |
| PDCoV-5′-R | CTACCTAGGCTCGGTGCAAGG |
| PDCoV-3′-F | CTTGCACCGAGCCTAGGTAGCTTGCAGGGATTATGGATCCAATGGGTACATG |
| PDCoV-3′-R | TTGCTCCATCCCCCCTATAAGCCAATTTAATTTCCCC |
| HDV-F | ATAGGGGGGATGGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGTCGGCATGGCATC |
| BGH-R | GAGAAGCTTCCATAGAGCCCACCGCATCCCCAGC |
| A-F | TAGCACTCCTTGCACCGAGCCTAGGTAGGATAAAACCCCCTAC |
| A-R | GGCAAATTTAATGGCAGGAC |
| B-F | GTCCTGCCATTAAATTTGCC |
| B-R | GTTGTGAATCGATTTGCAAG |
| C-F | CTTGCAAATCGATTCACAAC |
| C-R | CAGAGTGCATCTATTGCTGC |
| D-F | GCAGCAATAGATGCACTCTG |
| D-R | ACTGCTGGAATTCCTCGTGG |
| E-F | CCACGAGGAATTCCAGCAGT |
| E-R | GCACCTCCATGTACCCATTGGATCCATAATCCCTGCAAGGAG |
| Clone-F | CCAAGTTGGTGATTACATTC |
| Clone-R | GCTGAGTCAGTGGTCACGCA |
| qPDCoV-nsp16-F | GCCCTCGGTGGTTCTATCTT |
| qPDCoV-nsp16-R | TCCTTAGCTTGCCCCAAATA |
| qGAPDH-F | ACATGGCCTCCAAGGAGTAAGA |
| qGAPDH-R | GATCGAGTTGGGGCTGTGACT |