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. 2021 Sep 28;14(10):986. doi: 10.3390/ph14100986

Figure 4.

Figure 4

Effects of Gardenia jasminoides water (GJW) and 70% ethanol (GJE) extracts on the secretion of cytokines in differentiated Th2 cells. Naïve CD4+ T cells were differentiated into Th2 cells over 6 days. (A) The frequency of differentiated Th2 cells (CD3+ CD4+ GATA3+ cells) was examined using flow cytometry after treatment with GJW or GJE. (B,C) The viability of the differentiated Th2 cells was analyzed using EZ-Cytox solutions at the indicated concentrations of GJW or GJE. (D–I) The cytokine secretion levels in Th2 cells were assessed using LEGENDplex and flow cytometric analysis of the cell culture media. The data are presented as means ± standard deviations (n = 3). *** p < 0.001 vs. control; # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. ConA. Con, control; ConA, concanavalin A; IL, interleukin.