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. 2021 Sep 28;14(10):986. doi: 10.3390/ph14100986

Figure 6.

Figure 6

Effects of Gardenia jasminoides water (GJW) and 70% ethanol (GJE) extracts on the production of superoxide dismutase-2 (SOD2) and uncoupling protein-2 (UCP2), and on the activation of the p38-mitogen-activated protein kinase (MAPK)-activating transcription factor-2 (ATF2) signaling pathway in human nasal epithelial cells. (A–F) Cells were treated with GJW and GJE (10, 30, and 100 μg/mL) or with MitoTEMPO (100 μM) along with 15 ng/mL IL-4/IL-13 for 1 h. Total cell lysates were analyzed via western blotting using specific antibodies for SOD2 (A,B), catalase (A,B), UCP2 (C,D), phospho-p38, p38, phospho-ATF2, and ATF2 (E,F); total β-actin served as a loading control. Images were quantified using ImageJ. Data are representative of three independent experiments and expressed as the means ± standard deviations; * p < 0.05 and *** p < 0.001 vs. control; # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. IL-4/IL-13. p-p38, phospho-p38; p-ATF2, phospho-ATF2.