Table 1.
Regression equation, linearity, LOD, LOQ, and contents of the three compounds.
| Compound | Linear Range (μg/mL) |
Regression Equation (y = ax + b) (a) |
r 2 | LOD (b) (μg/mL) |
LOQ (c) (μg/mL) |
Content (mg/g) | ||
|---|---|---|---|---|---|---|---|---|
| Slope (a) | Intercept (b) | 70% EtOH | Water | |||||
| Gardenoside | 3.125–100 | 12175 | 7278.8 | 0.9997 | 0.313 | 0.950 | 21.24 ± 0.08 | 3.59 ± 0.02 |
| Geniposide | 25–800 | 15204 | 93731 | 0.9996 | 0.347 | 1.053 | 272.64 ± 0.21 | 129.64 ± 0.49 |
| Crocin | 3.125–100 | 8965.4 | 3561.9 | 0.9999 | 0.124 | 0.375 | 8.89 ± 0.00 | 3.84 ± 0.03 |
(a)y = ax + b, y means peak area and x means concentration (μg/mL); (b) LOD (limit of detection): 3.3 × (standard deviation of the response/slope of the calibration curve); (c) LOQ (limit of quantification): 10 × (standard deviation of the response/slope of the calibration curve).