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Time profiles of 6′a-hydroxymethyl-monacolin J formation, which was catalyzed using M380, M371, and M697. TTN values were determined using HPLC after accomplishing the reactions at indicated times at 37 °C. The formation of 6′a-hydroxymethyl monacolin J was measured in the reaction mixtures containing 0.2 μM of P450, an NADPH regeneration system, and monacolin J (0.30 mM) for a total volume of 0.25 mL.
