Figure 2.

CPP/siRNA NPs induced therapeutic gene knockdown, RRM2 protein decrease, and cell cycle arrest in endometriotic cells. (A,B) RRM2 and (C,D) VEGF gene expression levels measured in (A,C) peritoneal and (B,D) ovarian endometriotic cells using qRT-PCR. Data were analyzed by 2^−ΔΔCT method using GAPDH as an internal control and normalized to relevant pure siRNA groups. Error bars represent SEM of n = 7–8 individual samples from independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, one-way ANOVA, Tukey post hoc. (E) Representative immunocytochemical staining of RRM2 (green) in endometriotic primary cells counterstained with DAPI (blue); scale bar: 100 µm. (F) Representative flow cytometry graphs of propidium-iodide-stained endometriotic peritoneal and ovarian cells. All of the data (A–F) were collected 48 h after transfection and representative images (E,F) are from 3 independent experiments. UT, untreated.