FIG. 3.

Analysis of PCR-amplified 116-bp fragment, by 2.5% agarose gel electrophoresis. DNA was extracted from blood samples (A) and lymph node aspirates (B). Details of PCR amplification, sequence of primers, cloning, and sequencing of probe are given in the text. Samples of 50 μl of PCR products were analyzed. (A) Lanes 1 to 12, 116-bp amplified fragment from blood samples. (B) Lanes 1 to 12, 116-bp amplified fragment from lymph node aspirates. (A and B) Lane 13, control without Leishmania DNA; lane 14, positive control amplified from 1 ng of total Leishmania DNA; lane 15, ladder 50 as a DNA molecular size marker.