FIG. 4.

Analysis of PCR products by dot blot hybridization with 11 dUTP-labeled 70-bp probe. Details of PCR amplification, sequence of primers, cloning, and sequencing of probe are given in the text. The samples were the same as those described in the legend to Fig. 3. Spots 1a to 6b, PCR products from blood samples; spots 1c to 6d, PCR products from lymph node aspirate samples; C−, control without Leishmania DNA; C+, target DNA amplified from 1 ng of total Leishmania DNA.