SD1 expression in wild‐type tissues and during SAM development. (a) Relative SD1 expression in tissues during rice development. Mean ± SE, n = 3. ml, mature leaf; yl, young leaf; yl sh, young leaf sheath; pbp, primary branch primordium; sbp, secondary branch primordium; el, elongating stem; pl, panicle length. (b–e) RNA in situ assay for SD1 expression (b) at rachis meristem stage; (c) primary branch primordium later stage and secondary branch primordium initial stage; (d) secondary branch primordium later stage; and (e) sense probe as negative control at primary branch stage. Bar = 100 μm. (f–i) GFP signal directed by the SD1 promoter at different stages of branch primordium development, including (f) rachis meristem stage; (g) primary branch primordium initial stage; (h) primary branch primordium later stage; (i) secondary branch primordium initial stage. Bar = 100 μm. rm, rachis meristem; pbm, primary branch meristem; ebpb, elongating primary branch meristem; sm, spikelet meristem.