Figure 1.

Development and application of an scRNA‐seq approach for the peanut leaf. (a) Brief process of isolation of peanut leaf protoplast cells and their loading onto the 10×Genomics platform. (b) Violin plots show the expression pattern and distribution of cluster‐specific genes. (c) Classification of distinct peanut leaf cell clusters (t‐SNE map). Each dot indicates individual cells that are coloured based on cell type. (d‐e) t‐SNE map embedding of sub‐cluster assignments following a second round of clustering within palisade and spongy tissues.