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. 2021 Jun 19;19(11):2177–2191. doi: 10.1111/pbi.13644

FIGURE 5.

FIGURE 5

Early endosperm and embryo development in WT and enl2‐2 seeds. (a and b) PI staining of whole‐mount developing WT (a) and enl2‐2 (b) ovaries at 3 HAP. Developing WT (a1 and a3) and enl2‐2 (b1 and b3) embryos. Developing WT (a2 and a4) and enl2‐2 (b2 and b4) endosperm. Scale bars, 50 µm (a1, a2, b1 and b2); 20 µm (a3, a4, b3 and b4). (c and d) PI staining of whole‐mount developing WT (c) and enl2‐2 (d) ovaries at 8 HAP. Developing WT (c1 and c3) and enl2‐2 (d1 and d3) embryos. Developing WT (c2 and c4) and enl2‐2 (d2 and d4) endosperm. Scale bars, 50 µm (c1, c2, d1 and d2); 20 µm (c3, c4, d3 and d4). (e and f) PI staining of whole‐mount developing WT (e) and enl2‐2 (f) ovaries at 24 HAP. Developing embryo of WT (e1 and e3) and enl2‐2 (f1 and f3). Developing WT (e2 and e4) and enl2‐2 (f2 and f4) endosperm. Scale bars, 100 µm (e1, e2, f1 and f2); 20 µm (e3, e4, f3 and f4). Arrows indicate critical differences at 24 HAP.