Figure 7.

Overexpression of PagCYP736A12 decreased oxidative damage levels under salt stress. (a) Representative DCFH‐DA staining to detect H₂O₂ in the root tips of non‐transgenic 84K and transgenic plants. (b, c) H₂O₂ and MDA contents in the fresh roots of non‐transgenic 84K and transgenic plants. The cutting‐propagated non‐transgenic 84K and transgenic poplar lines overexpressing PagCYP736A12 (OX1 and OX2) were cultivated and salinity treated with 0 and 75 mm NaCl as described in Figure 2. At least 20 roots were collected from three biological replicates (more than 10 individuals each) for DCFH‐DA staining and analysis of H₂O₂ and MDA content. FW, fresh weight. The experiments were performed three times and the similar results were obtained. The bars represent means ± SD (n = 6) and the asterisks indicate significant differences between non‐transgenic 84K and transgenic plants (*P < 0.05).