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. 2021 Aug 3;19(11):2319–2332. doi: 10.1111/pbi.13663

Figure 1.

Figure 1

The expression pattern of the OsRLP1 gene. (a) RT‐qPCR analysis of OsRLP1 gene expression levels in RBSDV‐infected plants and controls at 30 and 60 dpi. Values are the means ± SD from 3 biologically independent samples. *Significant difference at P < 0.05 from Fisher's LSD test. (b) Subcellular localization of OsRLP1 protein. OsRLP1‐eGFP was transiently expressed in N. benthamiana leaves with 35S::eGFP construct as negative control. The infiltrated‐tobacco leaves were stained with plasma membrane dye FM4‐64 for 10 min. Excitation laser wavelengths of 514 nm and 563 nm were used for GFP and FM4‐64 signals, respectively. The white square in the merged image is magnified as a detailed picture. White arrows indicate the region of interest (ROI) and intensity profiles show the pixels grey value across the ROI in the eGFP and RFP channels. White bar represents 20 μm.