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. 2021 Aug 3;19(11):2319–2332. doi: 10.1111/pbi.13663

Figure 3.

Figure 3

OsRLP1 positively regulates rice resistance to RBSDV. (a) The symptoms of RBSDV in WT (NIP), transgenic plants overexpressing OsRLP1 (OsRLP1) and mutant (osrlp1‐cas) plants. The phenotypes were photographed at 30 dpi. White bar represents 5 cm. (b) Disease incidence in NIP (control), OsRLP1 lines (18# and 23#) and osrlp1‐cas (1# and 2#) lines at 30 dpi. Disease incidence is the percentage of RBSDV‐infected plants based on the numbers of healthy and diseased plants as determined by RT‐PCR. (c) The relative expression levels of RBSDV S6, S7 and S10 genes in RBSDV‐infected NIP, OsRLP1 lines (18# and 23#) and osrlp1‐cas lines (1# and 2#) assessed by RT‐qPCR at 30 dpi. UBQ5 was used as the internal reference gene to normalize the relative expression. Values are the means ± SD from 3 biologically independent samples. *Significant difference at P < 0.05 from Fisher's LSD test. (d) The accumulation of RBSDV P10 protein in RBSDV‐infected plants determined by Western blotting at 30 dpi. Total proteins were stained with Coomassie brilliant blue (CBB).