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. 2021 Aug 3;19(11):2319–2332. doi: 10.1111/pbi.13663

Figure 8.

Figure 8

The effect of RBSDV infection on OsSOBIRI1 mutant plants. (a) The relative expression levels of the PTI‐ and MAPK‐related genes in response to flg22 in NIP plants as assessed by RT‐qPCR. NIP plants were infiltrated with 10 μm flg22, and leaves were collected 30 min post‐infiltration for transcript analysis. UBQ5 was used as the internal reference gene to normalize the relative expression. Values are the means ± SD from 3 biologically independent samples. *Significant difference at P < 0.05 from Fisher's LSD test. (b), (c) The relative expression levels of the PTI‐ and MAPK‐ related genes and MAPK in response to flg22 in ossobir1‐cas (4# and 5#) plants assessed by RT‐qPCR. ossobir1‐cas plants were infiltrated with 10 μm flg22, and leaves were collected 30 min post‐infiltration for transcript analysis. UBQ5 was used as the internal reference gene to normalize the relative expression. Values are the means ± SD of 3 biologically independent samples. *Significant difference at P < 0.05 from Fisher's LSD test. (d) The symptoms of RBSDV on infected WT (NIP) and ossobir1‐cas (4# and 5#) plants. The phenotypes were photographed at 30 dpi. White bar represents 5 cm. (e) Disease incidence in NIP (control) and ossobir1‐cas lines. Disease incidence is the percentage of RBSDV‐infected plants. (f) The relative expression levels of RBSDV S6, S7 and S10 genes in RBSDV‐infected NIP and ossobir1 mutant lines assessed by RT‐qPCR at 30 dpi. UBQ5 was used as the internal reference gene to normalize the relative expression. Values are the means ± SD from 3 biologically independent samples. *Significant difference at P < 0.05 from Fisher's LSD test. (g) The accumulation of RBSDV P10 protein in RBSDV‐infected NIP and ossobir1 mutant plants determined by Western blotting. Total proteins were stained with Coomassie brilliant blue (CBB) to show equal loading.