Figure 1.

Strategy of reducing the copy number of BnQCR8 in cultivar Westar of rapeseed. (a) The phylogenetic tree of eight homologous copies of BnQCR8 and QCR8 from other plants selected. The phylogenetic tree was made with the maximum‐likelihood algorithm. Branch lengths are proportional to the average probability of change for characters on that branch. The red pentagram showed the BnQCR8s in rapeseed. (b) The interaction between SsSSVP1^ΔSP and BnQCR8. The pGADT7‐SV40 tag co‐transferred with pGBKT7‐Lam pGBKT7‐p53 were used as negative and positive controls, respectively. Photos were taken 2 dpi. (c) The structure of BnQCR8 and the sgRNAs in cultivar Westar of rapeseed. The sgRNA1 and sgRNA2 sites are in the exon shown with red vertical line and the direction shown with the black arrow and the different base is shown in green. The PAM is in red. The sequences in the red box are the sgRNA1 and sgRNA2 in the vector. (d) The schematic of the T‐DNA region of the pRGEB35‐Cas9‐BnQCR8 vector. The two sgRNAs were driven by AtUBI10