Figure 4.

Interactions between BnQCR8 and its mutated BnQCR8 and BcSSVP1 of B. cinerea or SsSSVP1 of S. sclerotiorum. (a) Yeast two‐hybrid (Y2H) assays showed the interaction between BcSSVP1^ΔSP and BnQCR8 (BnQCR8.A10) or AtQCR8. pGADT7‐SV40 TAg co‐transferred with pGBKT7‐Lam or pGBKT7‐p53 was used as negative and positive controls. SD–Ade/–His/–Leu/–Trp contains125 ng mL⁻¹ aureobasidin A (ABA) and 40 μg mL⁻¹ X‐α‐Gal was used. Photos were taken 2 dpi. (b) Interaction of edited BnQCR8 with SsSSVP1^ΔSP by Yeast two‐hybrid (Y2H) assays. “+A” indicate the insertion of adenine. D1 and D3 indicate one and three bases deletions, respectively. (c) The termination and frame shift amino acid site of the edited copies of BnQCR8, and ‘none’ indicates no events occurred in the edited copies of BnQCR8