Figure 2.

Screening of the Sw‐5b^R927A mutagenesis library led to the selection of two candidates, Sw‐5b^mutant‐137 and Sw‐5b^mutant‐1665, that gained HR to NSm^C118Y and NSm^T120N from TSWV RB isolates. (a) Sw‐5b^mutant‐137 and Sw‐5b^mutant‐1665 were co‐expressed with or without the WT NSm, NSm^C118Y or NSm^T120N in N. benthamiana leaves. Co‐expression of WT Sw‐5b and NSm was used as a positive control. The HR phenotypes were monitored at 3‐10 dpi and photographed at 7 dpi. (b) Amino acid mutations in the SD domain of Sw‐5b^mutant‐137 and Sw‐5b^mutant‐1665. The amino acid sequences of the SD domain of WT Sw‐5b, Sw‐5b^mutant‐137 and Sw‐5b^mutant‐1665 were aligned using the ClustalW program. Amino acid differences at residues 33 (L substituted to P) and 319 (K to L) among the WT Sw‐5b, Sw‐5b^mutant‐137 and Sw‐5b^mutant‐1665 are boxed and indicated in red.