FIG. 10.

Muscle differentiation assay showing blockage of NotchIC function by antisense SKIP. Photomicrographs of C2C12 and C2C12-derived cell lines in growth medium (a, c, e, and g) or after 6 days in differentiation medium (b, d, f, and h). In differentiation medium, C2C12 cells fused to form myotubes (b) whereas the CDN2 cells, which constitutively express Notch2IC, continued to grow as undifferentiated myoblasts (d). In antisense-SKIP-expressing CDN2-asSKIP cells, myotubes formed that had the same morphology as those formed by C2C12 cells (f). This differentiation was not observed in sense-SKIP-expressing CDN2-SKIP cells (h).