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SKIP mediates transcriptional repression. Shown are results of a transient-expression assay demonstrating repression of the 5× Gal4TK-CAT reporter by Gal4-SKIP fusion proteins. SKIP, C. elegans SKIP (CeSKIP), and Drosophila SKIP (DmSKIP) expressed as fusions with Gal4(1–147) were cotransfected into HeLa cells with the 5× Gal4TK-CAT reporter (0.8 μg) and 1 μg of TK-luciferase as a control for transfection efficiency.
