FIG. 8.

NotchIC mutated in the fourth ankyrin repeat has impaired ability to activate a reporter containing CBF1 binding sites. (A) Transient-expression assay of HeLa cells cotransfected with 8× wtCBF1 BS-Luc and either wild-type NotchIC (wtN1IC) or an ankyrin repeat NotchIC mutant [N1IC(ank)] as indicated. (B) Transient-expression assay performed as described for panel A but with 8× mtCBF1 BS-Luc. The reporter carrying mutated CBF1 binding sites was not responsive to wild-type or mutant NotchIC.