Figure 4. Template switching is common in both COs and NCOs.
(A) Multiple rounds of invasion, extension, and displacement can form mosaic hybrid DNA.
(B) Examples of mosaic heteroduplex among NCOs and COs. Black bars–minimum extent of mosaic heteroduplex. The NCO first invaded the homolog, while the CO first invaded the sister chromatid.
(C) Frequent template switching in COs and NCOs. Inset—fraction of tetrads with template switching. Tetrads where interpretation was uncertain are counted as “no template switch”. Main graph—number of template switches per NMS half-tract.
(D) Length of segments in NMS half-tracts with or without template switching. Red lines-median and quartiles. None of the distributions are significantly different from the others (p>0.05, Mann-Whitney test).