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. Author manuscript; available in PMC: 2022 Nov 1.
Published in final edited form as: Cell Calcium. 2021 Aug 31;99:102467. doi: 10.1016/j.ceca.2021.102467

Figure 1: TRPV4 channel activity is altered in the presence of IGF-1.

Figure 1:

A) Representative IV curve ATDC5 cells during a voltage ramp conducted from −80mV to +80mV that were challenged with hypotonic swelling (HS, red) after treatment with the IGF-1 (300ng/mL) for 3 hours (blue), or static controls (grey). Current was greatly reduced when cells were pretreated with IGF-1. B) Mean whole cell conductance of TRPV4 in nS is shown during a voltage ramp from −80mV to +80mV. Cells were static controls (black), untreated cells (grey), or cells pretreated with IGF-1 (light grey) or the TRPV4 antagonist, RN1734 (10μM, white) for 15 minutes, before challenging with HS. IGF-1 treatment and inhibition of TRPV4 completely abolished the conductance in cells through TRPV4 when compared to cells challenged with HS alone. (Tukey-Kramer; *** indicates p-value is < 0.001 in comparison to all other conditions, error bars = ±SEM).