Skip to main content
. Author manuscript; available in PMC: 2022 Nov 1.
Published in final edited form as: Cell Calcium. 2021 Aug 31;99:102467. doi: 10.1016/j.ceca.2021.102467

Figure 2: IGF-1 increases actin stress fiber formation and cell stiffness among ATDC5 cells.

Figure 2:

A) Time course of IGF-1 treatment indicates an increased stress fiber formation (white arrows) and cell spreading after 1 hour, with the peak amount occurring after 3 hours of treatment. Bar indicates 50 μm. B) Apparent cell stiffness of ATDC5 cells was significantly higher after 3 hours of treatment with IGF-1. The increased stiffness due to IGF-1 was dependent on F-actin organization based on the decrease in cell stiffness following cytochalasin D. (* indicates p-value is < 0.05 compared to controls)