Figure 3: The [Ca²⁺]_i response to HS is mediated through TRPV4 channel.

A) The number of cells responding and B) the change in [Ca²⁺]_i as a percentage of baseline when ATDC5 cells were treated with 10 μM ruthenium red prior to and during hypotonic swelling (HS + RR) in order to inhibit all TRPV channels. Similar results were observed when only TRPV4 was inhibited by treating ATDC5 cells with 10 μM RN1734 prior to and during hypotonic swelling (HS + RN). (* indicates p-value < 0.05 compared to controls, error bars = ± SEM)