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. 2021 Oct 23;12(11):989. doi: 10.1038/s41419-021-04272-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Immunohistochemistry of METTL3 in 3-week-old mouse ovaries at different follicle stages with PMSG injection. The arrows indicate oocytes at different follicle stages. Scale bar, 20 μm. B Confocal immunofluorescence images of oocytes from wild-type mice stained with METTL3 antibody (red) and DAPI (blue), as indicated. PD5 postnatal days 5, PD12 postnatal days 12, GV germinal vesicle, MII metaphase II. Scale bar, 20 μm. C qRT-PCR analysis of Mettl3 mRNA levels in oocytes from 3-week-old WT and Mettl3^Gdf9 cKO females. The relative mRNA level of Mettl3 in WT oocytes was set to 1.0. ***p < 0.001 by two-tailed Student’s t-test. Data represent the mean ± SEM (n = 3). D Immunohistochemistry for METTL3 in ovary sections from WT and Mettl3^Gdf9 cKO mice. Primordial (PrF) and secondary (SF) follicle stages are indicated. Scale bar, 20 μm. E Immunoblotting analysis of METTL3 protein level in oocytes of WT and Mettl3^Gdf9 cKO mice. GAPDH was used as an internal control. One hundred germinal vesicle oocytes were used for each lane of the blots. F Cumulative numbers of pups born from six pairs of WT and Mettl3^Gdf9 cKO female mice for 5 months. G Representative images of ovaries from 6-week-old, 8-week-old, and 12-week-old female mice. Scale bar, 200 μm. H Ratio of ovary weight to body weight of 6-week-old, 8-week-old, and 12-week-old female mice. 6-week-old, n = 3; 8-week-old, n = 6; 12-week-old, n = 6. n.s., p > 0.05; ***p < 0.001 by two-tailed Student’s t-test. Data represent the mean ± SEM.