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. 2021 Oct 23;10(10):70. doi: 10.1038/s41389-021-00362-7

Fig. 1. ACTL6A maintains the aggressive cancer phenotype.

Fig. 1

A ACTL6A was successfully knocked down using ACTL6A-siRNA which leads to an increase in p21Cip1 protein level. B–D ACTL6A knockdown decreases spheroid number and size, invasion and migration. For spheroid assay, 40,000 cells were seeded on ultra-low attachment plates and counted at Day 4. Spheroid diameters were measured using ImageJ. E–H ACTL6A knockout cell clones, which lack ACTL6A, display elevated p21Cip1 expression and this is associated with reduced cell proliferation, spheroid formation and invasion. I, J ACTL6A overexpression in ACTL6A knockout cell clones reduces p21Cip1 protein and mRNA levels. Asterisks indicate a significant decrease (n = 3, p ≤ 0.001).