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. 2021 Oct 23;12(11):992. doi: 10.1038/s41419-021-04283-6

Fig. 2. Deletion of soluble epoxide hydrolase (sEH) ameliorated high phosphate (Pi)-induced vascular smooth muscle cell (VSMC) calcium deposition and phenotypic transition.

Fig. 2

A Alizarin Red S staining of VSMCs. B The related OD value (562 nm) of Alizarin Red S staining. C Quantification of calcium contents of VSMCs. The calcium contents of each group were normalized to the related protein concentrations. Data are presented as mean ± SD. *P < 0.05 vs. wild-type (WT) 0d; #P < 0.01 vs. WT 0 d; &P < 0.0001 vs. WT 0 d. D–H Representative western blot bands (D), and the quantitative analysis of bone morphogenetic protein-2 (BMP2) (E), runt-related transcription factor 2 (Runx2) (F), alpha smooth muscle actin (α-SMA) (G), and smooth muscle 22 alpha (SM22α) (H) in VSMCs. Data are presented as mean ± SD. *P < 0.05; #P < 0.01; $P < 0.001; &P < 0.0001.