Table 1. The hydrolysis conditions for the preparation of protein hydrolysates from Chinese pond turtle muscle.
Hydrolysis condition | Protease | |||
---|---|---|---|---|
Alcalase | Flavourzyme | Trypsin | Bromelain | |
Incubation temperature/°C | 55 | 50 | 60 | 55 |
pH | 8.0 | 7.5 | 8.0 | 7.0 |
(m(enzyme)/m(substrate))/% | 1, 2, 3, 4, 5 | 1, 2, 3, 4, 5 | 1, 2, 3, 4, 5 | 1, 2, 3, 4, 5 |
(m(solid)/V(liquid))/(g/mL) | 1:1 | 1:1 | 1:1 | 1:1 |
t(incubation)/h | 1, 3, 5, 7 | 1, 3, 5, 7 | 1, 3, 5, 7 | 1, 3, 5, 7 |
Inactivation temperature/°C | 90 | 90 | 90 | 90 |
t(inactivation)/min | 20 | 20 | 20 | 20 |