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. 2021 Sep;59(3):360–375. doi: 10.17113/ftb.59.03.21.7087

Table 1. The hydrolysis conditions for the preparation of protein hydrolysates from Chinese pond turtle muscle.

Hydrolysis condition Protease
Alcalase Flavourzyme Trypsin Bromelain
Incubation temperature/°C 55 50 60 55
pH 8.0 7.5 8.0 7.0
(m(enzyme)/m(substrate))/% 1, 2, 3, 4, 5 1, 2, 3, 4, 5 1, 2, 3, 4, 5 1, 2, 3, 4, 5
(m(solid)/V(liquid))/(g/mL) 1:1 1:1 1:1 1:1
t(incubation)/h 1, 3, 5, 7 1, 3, 5, 7 1, 3, 5, 7 1, 3, 5, 7
Inactivation temperature/°C 90 90 90 90
t(inactivation)/min 20 20 20 20