FIG. 5.

hPGC-1 interacts functionally with the LBD of the receptor. COS7 cells were transfected with expression plasmids for either full-length GR (N795) (aa 1 to 795), the truncated GR variant N525 (aa 1 to 525) or 407C (aa 407 to 795), or the chimeric activator Gal4-LBD (aa 525 to 795 of GR); the GR- or Gal4-responsive reporter pTAT3Luc or pGK-1, respectively; and either vector alone (hatched bars) or the hPGC-1 expression vector (black bars). Cells were treated with 50 nM corticosterone (+) or carrier ethanol (−) for 24 h and assayed for luciferase activity. Data are the mean ± standard deviation luciferase units of eight transfections from four independent experiments.