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The C terminus of hPGC-1 interacts with itself. Yeast carrying a Gal4-responsive β-gal reporter and expressing Gal4-294C was transformed with vectors for the Gal4 AD, either alone (AD) or fused to full-length and truncated hPGC-1 variants as indicated, and assayed for β-gal activity. hPGC-1 variants are named as in Fig. 6 and 7. Data are the mean ± standard deviation β-gal activity of six or more independent yeast transformants.
