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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: J Nutr Biochem. 2020 Dec 31;89:108580. doi: 10.1016/j.jnutbio.2020.108580

Figure 1. WBM extract causes a dose-dependent decrease in cell proliferation, cellular AR, and PSA levels.

Figure 1.

Cells were treated with the mushroom extract (WBM, 1 μL/mL~5 μL/mL) or enzalutamide (Enza, 5 μM) supplied with 1 nM DH for 12~48h. The proliferation of A) LNCaP and B) VCaP cells was measured by the CyQuant NF Cell Proliferation reagent. The PSA production in the culture medium of C) LNCaP and D) VCaP cells was detected by PSA (human) ELISA kit. Total protein of AR and PSA in E) LNCaP and F) VCaP cells was detected by western blot, and mRNA of AR and PSA in G) LNCaP and H) VCaP cells was quantified by qRT-PCR. The results are mean ± standard deviation of three independent experiments, p values were determined by multiple Student’s t-tests. *p<0.05, **p<0.01, ***p<0.001. ns. No significant difference.