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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: J Nutr Biochem. 2020 Dec 31;89:108580. doi: 10.1016/j.jnutbio.2020.108580

Figure 2. WBM extract interrupts AR expression, AR nuclear-cytoplasmic distribution, and transactivation.

Figure 2.

Cells were treated with the mushroom extract (WBM, 1 μL/mL~5 μL/mL) or enzalutamide (Enza, 5 μM) supplied with 1 nM DHT for 48h. The AR cellular localization in A) LNCaP and B) VCaP cells was determined by immunofluorescence staining. Magnification at 20X and 100X with a scale bar of 100 μm and 20 μm, respectively. The protein amount of AR in the cytoplasmic and nuclear fractions of C) LNCaP and E) VCaP cells was determined by western blot. GAPDH and nucleolin were used as the cytoplasmic and nuclear loading controls, respectively. The AR reporter activity in D) LNCaP and F) VCaP cells was determined by PSA promoter-Luciferase assay. **p<0.01. ns. No significant difference.