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. 2021 Oct 24;14:89. doi: 10.1186/s12284-021-00530-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Expression patterns of OsLCT2. a–c The expression of OsLCT2 was determined by qRT-PCR. a Tissue-dependent expression of OsLCT2 in 2-week-old seedlings. The leaf, root and basal stem (2 cm above the roots) were sampled for expression analysis. Relative expression of OsLCT2 is shown, with its expression in the basal stem set to 1. b Response of OsLCT2 in seedling roots to the absence or excess of Fe, Mn or Zn. Seven-day-old seedlings were grown in Yoshida nutrient solution without Fe, Mn, or Zn or with high concentrations of Fe, Mn, or Zn for 7 d. Seedlings grown in standard Yoshida nutrient solution were used as controls. c Response of OsLCT2 in seedling roots to external Cd. Two-week-old seedlings were exposed to different concentrations of Cd for 24 h. Data show means ± SD of three biological replicates. In b and c, asterisks above the bars indicate significant differences between the treatments and controls (*P < 0.05, **P < 0.01; Student’s t-test). d Histochemical GUS staining of roots of transgenic rice plants transformed with OsLCT2 promoter-driven GUS constructs. Tissue-specific expression of OsLCT2 in the entire roots (I), root tip (II), lateral roots (III), root elongation zone (IV, V), and maturation zone (VI, VII) visualized in cross section (IV, VI) and vertical section (V, VII)