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. Author manuscript; available in PMC: 2022 Oct 21.
Published in final edited form as: Cell Chem Biol. 2021 May 26;28(10):1501–1513.e5. doi: 10.1016/j.chembiol.2021.05.001

Figure 4. TgASH4 deletion alters the T. gondii lipid profile.

Figure 4.

(A) Image of TLC separation of lipids extracted from wild type, ΔTgAsh4, ΔTgAsh[ASH4] and ΔTgAsh4 TgASH4 [S124A] parasites. Lipids were stained by cupric sulfate. (B) Quantification of stained lipids from TLC analysis and plotted as a log2fold change. Box plots showing lipid distribution in ΔTgAsh4 relative to wild type parasites grouped by (C) main general categories of lipids and (D) the subclass of glycerophospholipids with log2fold changes indicated. (E) schematic of glycerolipid metabolism pathways, with numbers in red indicating log2fold change in ΔTgAsh4 vs wild type. Abbreviations: Cytidine-diphosphate-diacylglycerol (CDP-DAG), Phosphatidic acid (PA), Phosphoinositol (PI), Phosphatidyl-glycerol (PG), Phosphatidylserine (PS), Phosphatidyl ethanolamine (PE), Phosphatidyl choline (PC), Triacylglycerol (TAG), Diacylglycerol (DAG), Monoacylglycerol (MAG), Glycerol 3-phosphate (Glycerol-3-P), Phosphatidyl-glycerol-phosphate (PGP), Cardiolipin (CL), Dihydroxyacetone-phosphate (DHAP), Cytidine-diphosphate-choline (CDP-choline), Cytidine-diphosphate-ethanolamine (CDP-Ethanolamine) (see also Figure S2).